Phalloidin-iFluor 680 Reagent | CytoPainter (ab176760) is one of a series of phalloidin conjugates that selectively binds to actin filaments, also known as F-actin. The iFluor 680 dye can be easily detected with a fluorescent microscope at Ex/Em = 650/665 nm (NIR range). Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.